Lab
A summary of the Petriz lab research interests

In the last ten years I was involved in two main research projects on human stem cells. A number of data point to the existence of different classes of human stem cells with variable self-renewal potential and short- or long-term repopulating capacity. These observations led us to investigate the telomere length in highly purified human hematopoietic CD34+ cells, obtained from the mobilized peripheral blood of healthy donors, and from patients with multiple sclerosis (MS) undergoing autologous stem cell transplantation. We used flow cytometry-based fluorescent in situ hybridization (Flow-FISH) for the measurement of relative telomere length. We showed that a very small fraction of cells with very large telomeres was systematically identified in all samples of purified CD34+ cells, lending support to previous observations on the heterogeneity of the stem cell compartment

(FACS-based isolation of HSCs using Flow-FISH, in association with the mRNA expression analysis of gene expression in highly purified preparations of stem cell subsets on the basis of telomere length).

1301abc familySPs

More recently, I started my research on the Side Population (SP) stem cells. ABCG2, a half-transporter that belongs to the ABC transporter superfamily, is expressed in primitive stem cells, and is responsible for the formation of a SP with a Hoechst 33342 fluorescent profile blocked in the presence of multidrug reversal agents. SP cells are present in a wide variety of tissues and ABCG2 expression is believed to represent a common molecular mechanism for stem cells possessing multi-organ plasticity. FACS-based isolation of SP cells, in association with the mRNA expression analysis of gene expression in highly purified preparations of stem cell subsets -on the basis of ABCG2 expression- is providing us important insights in SP biology. I am studying the single-cell gene expression profile of SP cells to relate the expression of specific genes to a particular cellular phenotype

(FACS-based isolation of normal and cancer stem cells originated from the Side Population in association with the gene expression in highly purified preparations of stem cell subsets).